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Publications by Application:
Reviews by Application:
Publications and Posters
Adenoviral Transduction
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Amniotic Epithelial Cells
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BoNT/A
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Cell Based Assays
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Cell Migration
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References |
ArunA Product |
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Development of a novel high throughput screening/high content imaging amenable assay for cell proliferation and migration in human embryonic stem cell derived neural progenitors
Powe, A et al. Society for Neuroscience 2011.
(poster presentation)
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hNP1™
AB2™ ANS™
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Development of a Novel HTS Amenable Assay Platform for Visual Assessment of Human Embryonic Stem Cell Derived Neural Progenitor Migration
Powe, A et al. ISSCR 2011.
(poster presentation)
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hNP1™
AB2™ ANS™
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A Novel HTS-Adaptable Assay for Cell Migration of hESC-Derived Progenitors
Powe, A et al. HH Workshop 2011.
(poster presentation)
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hNP1™
AB2™ ANS™
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Development of a Novel Assay Platform for Visual Assessment of hESC Derived Neural Progenitor Cell Migration in Response to Neurotoxicants
Powe, A, et al. SOT 2011.
(poster presentation)
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hNP1™
AB2™ ANS™
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Identification of Stimulators and Inhibitors of Cell Migration in Human Embryonic Stem Cell Derived Neural Progenitors Using a Novel, High Throughput Amenable Assay Platform
Powe, A, et al. ASCB 2010.
(poster presentation)
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hNP1™
AB2™ ANS™
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Effects of Topography on the Functional Development of Human Neural Progenitor Cell
Wu ZZ, et al. Biotechnol Bioeng. 2010 Jul 1;106(4):649-59.
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hNP1™
AB2™ ANS™
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Characterization of Micropatterned Nanofibrous Scaffolds for Neural Network Activity Readout for High-Throughput Screening
Wang L, Kisaalita WS. J Biomed Mater Res B Appl Biomater. 2010 Jul;94(1):238-49.
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hNP1™
AB2™ ANS™
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Human Neural Progenitors Express Functional Lysophospholipid Receptors that Regulate Cell Growth and Morphology
Hurst JH, et al. BMC Neurosci. 2008 Dec 11;9:118.
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hNP1™
AB2™ ANS™
hN2™ |
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Cell Morphology
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Cell Proliferation
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Cell-Cycle Alterations
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Cellular Phenotyping
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Cellular Reprogramming
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References |
ArunA Product |
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Proliferative, Adherent Neural Progenitors Derived from Human Induced Pluripotent Stem Cells
Chilton, JM, et al. SENN&GASNC 2010
Conference Abstract, Published on Mar 2010 Front.
Neurosci. doi: 10.3389/conf.fnins.2010.04.00030
(poster presentation)
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hNP1™
AB2™ ANS™
viPS™ Vector Kit
hMPro™
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High-Efficiency Induction of Neural Conversion in hESCs and hiPSCs with a Single Chemical Inhibitor of TGF-beta Superfamily Receptors
Zhou J, et al. Stem Cells. 2010 Aug 23. [Epub ahead of print]
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viPS™ Vector Kit |
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Porcine Induced Pluripotent Stem Cells Produce Chimeric Offspring
West FD, et al. Stem Cells Dev. 2010 Aug;19(8):1211-20.
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viPS™ Vector Kit |
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Proliferative, Adherent Neural and Mesenchymal Progenitors Derived from Human Induced Pluripotent Stem Cells
Chilton, JM et al. ISSCR 2010
(poster presentation)
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viPS™ Vector Kit
hNP1™
AB2™ ANS™
hMPro™
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Generation of Human Induced Pluripotent Stem Cells from Amniotic Epithelial Cells
William L. Blake, Pfizer Corporation, et al ISSCR 2010
(poster presentation)
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viPS™ Vector Kit |
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Formation of Human Induced Pluripotent Stem Cells is Accelerated Using Six Reprogramming Factors
Chilton, Jm et al. ISSCR 2009
(poster presentation)
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viPS™ Vector Kit |
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Differentiation and Adhesion
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Differentiation Capacity
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Differentiation Markers
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DNA Damage and Repair
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DNA Methylation
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Dopaminergic Differentiation
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Electrophysiology
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Epigenetic Regulation
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Flow Cytometry
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Folding
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Genetic Modification
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References |
ArunA Product |
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Nonviral Gene Delivery in Neural Progenitors Derived from Human Pluripotent Stem Cells
Dhara, Sujoy K. et. al. Methods in Mol Biology, 2011, Vol. 767, Part 5, 343-354, doi: 10.1007/978-1-61779-201-4_2
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hNP1™
AB2™ ANS™
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Proliferative, adherent neural progenitors derived from human induced pluripotent stem cells
Chilton, JM, et al. SENN&GASNC 2010
Conference Abstract, Published on Mar 2010 Front.
Neurosci. doi: 10.3389/conf.fnins.2010.04.00030
(poster presentation)
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hNP1™
AB2™ ANS™
viPS™ Vector Kit
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Genetic manipulation of neural progenitors derived from human embryonic stem cells
Dhara SK, et al. Tissue Eng Part A. 2009 Nov;15(11):3621-34.
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hNP1™
AB2™
ANS™
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Adenoviral transduction of mesenchymal stem cells
Bosch P, Stice SL. Methods Mol Biol. 2007;407:265-74.
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hMPro™ |
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Formation of Human Induced Pluripotent Stem Cells is Accelerated Using Six Reprogramming Factors
Chilton, Jm et al. ISSCR 2009
(poster presentation)
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viPS™ Vector Kit |
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Germ Cell Isolation
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Germ Cells
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Glutamate (AMPA, Kainate) Receptor Pharmacology
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References |
ArunA Product |
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High-throughput screening in embryonic stem cell-derived neurons identifies potentiators of alpha-amino-3-hydroxyl-5-methyl-4-isoxazolepropionate-type glutamate receptors
Summary: Mouse vs Human neural cells produce different results in drug discovery assay. Mouse ESC and ArunA neural cells derived from human ESC were used in a FLIPR high throughput assay for AMPA potentiation compound testing. Both cells generated repeatable and robust cells however results did differ. Table 1 summarizes the results between the two species. The authors conclude ….”recent advances suggest that hES cell-derived neurons can be reproducibly scaled and quality-controlled during differentiation to consider moving directly to primary HTS in human cells …. this may eliminate translational differences between murine and human cellular generated data, as was observed in this study where 4/21 hits showed a 10 fold affinity difference between mouse and human cells.”
McNeish J, et al. J Biol Chem. 2010 May 28;285(22):17209-17.
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hNP1™
AB2™ ANS™
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Glycan Expression
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GPCR
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High-Content Imaging
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High-Throughput Screening
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Human Neural Progenitor Cells
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Human Lung Fibroblast
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Immuno-cytochemistry
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Induced Pluripotent Stem Cells
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References |
ArunA Product |
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Proliferative, Adherent Neural Progenitors Derived from Human Induced Pluripotent Stem Cells
Chilton, JM, et al. SENN&GASNC 2010
Conference Abstract, Published on Mar 2010 Front.
Neurosci. doi: 10.3389/conf.fnins.2010.04.00030
(poster presentation)
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hNP1™
AB2™ ANS™
viPS™ Vector Kit
hMPro™
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Human Induced Pluripotent Stem Cells Differentiate Into Osteo- And Chondrogenic Progenitors
Chilton, JM et al. ISSCR 2011
(poster presentation)
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viPS™ Vector Kit
hMPro™
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Osteo/Chondrogenic Progenitors Derived from Human Induced Pluripotent Stem Cells
Chilton, JM et al. HH Workshop 2011
(poster presentation)
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viPS™ Vector Kit
hMPro™
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High-Efficiency Induction of Neural Conversion in hESCs and hiPSCs with a Single Chemical Inhibitor of TGF-beta Superfamily Receptors
Zhou J, et al. Stem Cells. 2010 Aug 23. [Epub ahead of print]
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viPS™ Vector Kit |
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Porcine Induced Pluripotent Stem Cells Produce Chimeric Offspring
West FD, et al. Stem Cells Dev. 2010 Aug;19(8):1211-20.
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viPS™ Vector Kit |
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Proliferative, Adherent Neural and Mesenchymal Progenitors Derived from Human Induced Pluripotent Stem Cells
Chilton, JM et al. ISSCR 2010
(poster presentation)
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viPS™ Vector Kit
hNP1™
AB2™ ANS™
hMPro™
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Generation of Human Induced Pluripotent Stem Cells from Amniotic Epithelial Cells
William L. Blake, Pfizer Corporation, et al ISSCR 2010
(poster presentation)
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viPS™ Vector Kit |
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Formation of Human Induced Pluripotent Stem Cells is Accelerated Using Six Reprogramming Factors
Chilton, Jm et al. ISSCR 2009
(poster presentation)
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viPS™ Vector Kit |
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in vivo Modeling
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Ion Channel
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Lectin Binding
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Lentiviral Transduction
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Mesenchymal Differentiation
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Mesenchymal Progenitor Isolation
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Micro RNA Regulation
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Mitochondrial Gene Replacement
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Membrane Proteins
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Neural Development
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Neural Differentiation
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References |
ArunA Product |
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High Content Analysis of Neural Stem Cell Expansion and Differentiation
Sirenko, O et al. Society for Neuroscience 2011.
(poster presentation)
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hNP1™
AB2™ ANS™ |
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Nonviral Gene Delivery in Neural Progenitors Derived from Human Pluripotent Stem Cells
Dhara, Sujoy K. et. al. Methods in Mol Biology, 2011, Vol. 767, Part 5, 343-354, doi: 10.1007/978-1-61779-201-4_2
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hNP1™
AB2™ ANS™
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Differing Lectin Binding Profiles among Human Embryonic Stem Cells and Derivatives Aid in the Isolation of Neural Progenitor Cells
Dodla, Mahesh C. et. al. PLoS ONE. 2011 Aug 5. 6(8): e23266. doi:10.1371/journal.pone.0023266 |
hNP1™
hMPro™
AB2™ ANS™
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High Content Analysis of Neural Stem Cell Expansion and Differentiation
Sirenko, O et al. ISSCR 2011
(poster presentation)
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hNP1™
AB2™ ANS™
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Ion channels and ionotrophic receptors in human embryonic stem cell derived neural progenitors
Summary: In this study ArunA neural progenitor (hNP1™) cells and cells differentiated from the hNP1™ cells were characterized under non directed basal conditions where bFGF was removed from the culture medium. Using these same basal (low cost) conditions ArunA hNP1™ cells under whole-cell voltage clamp exhibited delayed rectifier potassium channel currents and exhibited tetrodotoxin sensitive, voltage-dependent sodium channel current. Although previous studies have demonstrated functional electrophysiological properties when hESC cells are differentiated to neural cells these prior studies used expensive cocktails of growth factors in extensive cultures. ArunA hNP1™ cells can be used in higher throughput electrophysiological studies using a minimal cost format.
Young A, et al. Neuroscience. 2011 Sep 29;192:793-805. Epub 2011 Jun 7.
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hNP1™
AB2™ ANS™
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Proliferative, adherent neural progenitors derived from human induced pluripotent stem cells
Chilton, JM, et al. SENN&GASNC 2010
Conference Abstract, Published on Mar 2010 Front.
Neurosci. doi: 10.3389/conf.fnins.2010.04.00030
(poster presentation)
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hNP1™
AB2™ ANS™
viPS™ Vector Kit
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Neural differentiation of human embryonic stem cells at the ultrastructural level
Mumaw JL, et al. Microsc Microanal. 2010 Feb;16(1):80-90. Review.
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hNP1™
AB2™ ANS™
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Stage-specific role for shh in dopaminergic differentiation of human embryonic stem cells induced by stromal cells
Swistowska AM, et al. Stem Cells Dev. 2010 Jan;19(1):71-82.
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hNP1™
AB2™ ANS™
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Highly efficient differentiation and enrichment of spinal motor neurons derived from human and monkey embryonic stem cells
Wada T, et al. PLoS One. 2009 Aug 24;4(8):e6722.
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hNP1™
AB2™ ANS™
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Endothelium-induced proliferation and electrophysiological differentiation of human embryonic stem cell-derived neuronal precursors
Lai B, et al. Stem Cells Dev. 2008 Jun;17(3):565-72.
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hNP1™
AB2™ ANS™
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Differentiation of Human Embryonic Stem Cell Derived Neural Progenitors into Functionally Responsive Populations in the Absence of Exogenous EGF
D.W. Machacek, et al. ISSCR 2008
(poster presentation)
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hN2™ |
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Development of Neural Cultures From Human Embryonic Stem Cell Derived Neural Progenitors
D. W. Machacek, et al. Society for Neuroscience 2007
(poster presentation)
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hN2™ |
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High-Efficiency Induction of Neural Conversion in hESCs and hiPSCs with a Single Chemical Inhibitor of TGF-beta Superfamily Receptors
Zhou J, et al. Stem Cells. 2010 Aug 23. [Epub ahead of print]
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viPS™ Vector Kit |
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Neural Progenitor Isolation
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Neural Proliferation
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Neurite Outgrowth
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References |
ArunA Product |
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Quantitative assessment of neurite outgrowth in human embryonic stem cell-derived hN2 cells using automated high-content image analysis
Summary: Thaw and use neuronal cells derived from hESC generates rapid and repeatable results in a neurite outgrowth high content imaging toxicity assay. ArunA hN2™ human embryonic stem cell derived neural cells were used in an automated high-content image analysis to measure neurite outgrowth in vitro. These thaw and use hN2™ cells generated neurites within six hrs and by 24 and 48 hrs there was a concentration-dependent decrease in neurite outgrowth and ATP-content were observed following treatment with either bisindolylmaleimide I, U0126, lithium chloride, sodium orthovanadate and brefeldin A (Figure 6). All of which have previously been shown to inhibit neurite outgrowth in primary rodent neural cultures. The authors suggest that this study “supports the hypothesis that measurements of neurite outgrowth are more sensitive than measures of cell health in the context of neurotoxicity screening… and hN2™ cells provide a model in which to investigate the processes of neurite outgrowth in the context of human biology.
Harrill JA, et al. Neurotoxicology. 2010 Jun;31(3):277-90.
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hN2™ |
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Comparative sensitivity of human and rat neural cultures to chemical-induced inhibition of neurite outgrowth
Summary: In an extension of the above study the ArunA hN2™ cells demonstrated that human neural cultures were more sensitive to neurite outgrowth inhibitors than primary rat cortical neuron cultures automated high-content image analysis to measure neurite outgrowth in vitro.
Harrill JA, et al. 2011. Toxico Appl Pharmacol. Epub 2011.
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hN2™ |
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Neurogensis
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Neuronal Differentiation
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Neurotoxicity
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Nucleofection
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Oxidative Stress
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Proliferation Rate
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Proteomics
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Quantitative PCR
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RNA Sequencing
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Splicing
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Stem Cell Transplantation
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Tissue Engineering
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Toxicology Screening
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References |
ArunA Product |
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Quantitative assessment of neurite outgrowth in human embryonic stem cell-derived hN2 cells using automated high-content image analysis
Summary: Thaw and use neuronal cells derived from hESC generates rapid and repeatable results in a neurite outgrowth high content imaging toxicity assay. ArunA hN2™ human embryonic stem cell derived neural cells were used in an automated high-content image analysis to measure neurite outgrowth in vitro. These thaw and use hN2™ cells generated neurites within six hrs and by 24 and 48 hrs there was a concentration-dependent decrease in neurite outgrowth and ATP-content were observed following treatment with either bisindolylmaleimide I, U0126, lithium chloride, sodium orthovanadate and brefeldin A (Figure 6). All of which have previously been shown to inhibit neurite outgrowth in primary rodent neural cultures. The authors suggest that this study “supports the hypothesis that measurements of neurite outgrowth are more sensitive than measures of cell health in the context of neurotoxicity screening… and hN2™ cells provide a model in which to investigate the processes of neurite outgrowth in the context of human biology.
Harrill JA, et al. Neurotoxicology. 2010 Jun;31(3):277-90.
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hN2™ |
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Transcriptional Profiling
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Transfection
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Review Articles
GPCR
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High-Throughput Screening
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Neural Differentiation
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Synapse Formation
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