Mi-T-MEF™ Mouse Feeder Cells

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Catalog: #A-6 | US $225.00 | International $250.00

Maintaining embryonic stem cells or induced pluripotent stem cells in a pluripotent, self-renewing and karyotypically normal state for a reasonable and defined period of time currently requires culturing ESC or iPSC in contact with established feeder cells such as mouse embryonic fibroblasts (MEFs).  ArunA Biomedical's Mi-T-MEF™ Mouse Feeder Cells are high quality cells that are mitotically inactivated through mitomycin C treatment and ready to use.  They have been prequalified for human embryonic stem cell work and provide an easy to use source of feeder layers to meet your stem cell needs.

 

 

 

Product Use Agreement
BIOLOGICAL PRODUCT(S) purchased and DERIVATIVE BIOLOGICAL MATERIALS resulting from the use of ArunA Biomedical BIOLOGICAL PRODUCT(S) shall not be: (i) used for any purpose other than internal research and internal research services, (ii) sold, reengineered and sold or otherwise provided to any third party for any use or purpose, (iii) provided to any agent or other third party for any reason whatsoever, or (iv) used for diagnostic or therapeutic purposes. Acceptance of these terms and conditions shall be deemed by RECIPIENT upon delivery of BIOLOGICAL PRODUCT(S) from ArunA Biomedical. 

Cell Type  primary mouse embryonic fibroblast cells
Part Number:  A-6
Species:  Mus musculus
Source:  ICR strain
Morphology:  fibroblast
Quantity:  > 8x106 cells per vial 

Shipping method:  dry ice
Storage:  liquid nitrogen
Growth Properties: adherent
Medium AB MEF Medium

Pathogen testing:    Product #A-6 has been tested for Hepatitis B, Hepatitis C, HIV-1, HIV-2, HTLV-I/II, HSV1, HSV2, EBV, and CMV and shown to be negative (performed by GIVF Laboratories). Cells are also tested for sterility and mycoplasma contamination.

Viability testing:  Post-cryostorage viability was determined to be approximately 75%. 

Kit format:

Mitomycin-C Inactivated Mouse Feeder Cells for Microenvironment Support to Pluripotent Stem Cells

  • Mi-T-MEF™ Mouse Feeder Cells (Cat#A-6), 1 vials (>8 x 106 cells)

Technical information:

Product Insert for Mi-T-MEF™ Mouse Feeder Cells (PDF)

Product Use Agreement
BIOLOGICAL PRODUCT(S) purchased and DERIVATIVE BIOLOGICAL MATERIALS resulting from the use of ArunA Biomedical BIOLOGICAL PRODUCT(S) shall not be: (i) used for any purpose other than internal research and internal research services, (ii) sold, reengineered and sold or otherwise provided to any third party for any use or purpose, (iii) provided to any agent or other third party for any reason whatsoever, or (iv) used for diagnostic or therapeutic purposes. Acceptance of these terms and conditions shall be deemed by RECIPIENT upon delivery of BIOLOGICAL PRODUCT(S) from ArunA Biomedical. 

Mi-T-MEF™ Mouse Feeder Cells can be used for studies of:

  • Microenvironment Support for Generation and Maintenance of Induced Pluripotent Stem Cells
     
  • Microenvironment Support for Generation and Maintenance of Human Germ Cells 
  • Microenvironment Support for Maintenance of Pluripotent Human and Mouse Embryonic Stem Cells

Mouse Feeder Cell references and publications can be found here.

Products are to be used for research purposes only, not for use in diagnostic or therapeutic applications. Products may not be sold, modified for sale or used for commercial purposes.

If you are having technical difficulties, please check our Frequently Asked Questions and/or contact us

I only obtained 50% recovery from the frozen feeder cells (Cat #A-6), what should I do?

Feeder density used for culturing pluripotent stem cells varies from lab to lab.  Feeders at a
density as low as 1 x 106 cells per 10 cm dish have been successfully used to culture human
embryonic stem (hES) cells (this is the density used by the Harvard Stem Cell Institute). Thus, you could still potentially use the feeder cells even if you got 50% recovery.  

Product Use Agreement
BIOLOGICAL PRODUCT(S) purchased and DERIVATIVE BIOLOGICAL MATERIALS resulting from the use of ArunA Biomedical BIOLOGICAL PRODUCT(S) shall not be: (i) used for any purpose other than internal research and internal research services, (ii) sold, reengineered and sold or otherwise provided to any third party for any use or purpose, (iii) provided to any agent or other third party for any reason whatsoever, or (iv) used for diagnostic or therapeutic purposes. Acceptance of these terms and conditions shall be deemed by RECIPIENT upon delivery of BIOLOGICAL PRODUCT(S) from ArunA Biomedical. 

 

 Left, Phase contrast image of mitomycin C inactivated AB Mi-T-MEF™ Mouse Feeder Cells (Cat# A-6) in culture. Right, Epiflourescent image of transcriptionally active AB MouseEZ™  following transduction with EF1α-TurboGFP lentiviral vector.