NeuroNet Pure Human Neurons Discovery Kit

add to cart
Catalog: hJL7014 | US $460 | Internationsl $690

The NeuroNet™ Pure Human Neurons are a highly pure population of hPSC derived neurons developed with versatility in mind. As a standalone model for studies of cellular and molecular neurobiology, drug discovery and neurotoxicity, NeuroNet™ Human Neurons enable rapid response in a relevant in vitro culture model system and have been strategically designed for co-planer cellular model studies with hAstroPro Human Astrocytes.

NeuroNet™ Pure Human Neurons can be cultured in an adherent 96-well format with ArunA’s proprietary culture medium and neural supplement and can be maintained in culture for longitudinal studies during which time NeuroNet cells have demonstrated extensive neurite outgrowth and synapse formation.

NeuroNet™ Pure Human Neurons are derived from WA09 human embryonic stem cells by ArunA Biomedical using a proprietary process under defined feeder-free & serum-free conditions. The cells are >95% β-III tubulin positive, >98% MAP2 positive, and free of hPSC. These differentiated neurons are cryopreserved for ready, convenient use.

NeuroNet™ Pure Human Neurons are ideal for use in co-culture with hAstroPro™Human Astrocytes

To request a custom quote or for bulk manufacturing contact us at 706-542-4941 or info@arunabiomedical.com.

The image below demonstrates NeuroNet™ Pure Human Neurons seeded on a 96 well plate prepped for neurite outgrowth assay.

The below image is one well of a 96-well plate at <48 hours post thaw and stained with Tuj1.

Product Use Agreement
BIOLOGICAL PRODUCT(S) purchased and DERIVATIVE BIOLOGICAL MATERIALS resulting from the use of ArunA Biomedical BIOLOGICAL PRODUCT(S) shall not be: (i) used for any purpose other than internal research and internal research services, (ii) sold, reengineered and sold or otherwise provided to any third party for any use or purpose, (iii) provided to any agent or other third party for any reason whatsoever, or (iv) used for diagnostic or therapeutic purposes. Acceptance of these terms and conditions shall be deemed by RECIPIENT upon delivery of BIOLOGICAL PRODUCT(S) from ArunA Biomedical. 

Cell Type:  Neuron
Part Number:  hJL7014
Species:  human
Source:  derived from human embryonic cell line, H9 (WA09)
Morphology:  neuronal
Quantity:  ~7x105 cells per vial
Shipping method:  dry ice
Storage:  liquid nitrogen
Growth Properties:  adherent, non-proliferative
Medium AB2™ Basal Neural Medium with ANS™ Neural Medium Supplement plus LIF (10 mg/mL)
Phenotyping:  Cells are >95% β III tubulin positive and >98% MAP2 positive.

DNA fingerprint:  Results are consistent with the presence of a single cell line with no evidence of culture cross contamination. The loci match the DNA fingerprint pattern for the H9 (NIH designation, WA09) hESC line as published in http://stemcells.nih.gov/research/nihresearch/scunit/.

Pathogen testing:   This lot was derived from the H9 hESC line that has been tested for Hepatitis B, Hepatitis C, HIV-1, HIV-2, HTLV-I/II, HSV1, HSV2, EBV, and CMV.  The H9 cell line has been tested and shown to be negative (performed by GIVF Laboratories).  Cells are tested for sterility and mycoplasma contamination.

Technical Information:

For additional product information, contact us at info@arunabiomedical.com or 706-542-4941

Product Use Agreement
BIOLOGICAL PRODUCT(S) purchased and DERIVATIVE BIOLOGICAL MATERIALS resulting from the use of ArunA Biomedical BIOLOGICAL PRODUCT(S) shall not be: (i) used for any purpose other than internal research and internal research services, (ii) sold, reengineered and sold or otherwise provided to any third party for any use or purpose, (iii) provided to any agent or other third party for any reason whatsoever, or (iv) used for diagnostic or therapeutic purposes. Acceptance of these terms and conditions shall be deemed by RECIPIENT upon delivery of BIOLOGICAL PRODUCT(S) from ArunA Biomedical. 

NeuroNet™ Pure Human Neurons have been derived from hPSC cell line WA09 (NIH 0062) and are used in a wide variety of basic research applications such as cellular and molecular neurobiology, drug discovery, and neurotoxicity.

 Applications for the hN2™ Differentiated Human Neuronal Cells include studies of:

Cell based compound screening
G-protein coupled receptors
Ion channels
Human Neurotoxicity Screening
Neural Progenitor Proliferation
Neurite Outgrowth

Neural stem cell references and reviews can be found here.

 

Product Use Agreement
BIOLOGICAL PRODUCT(S) purchased and DERIVATIVE BIOLOGICAL MATERIALS resulting from the use of ArunA Biomedical BIOLOGICAL PRODUCT(S) shall not be: (i) used for any purpose other than internal research and internal research services, (ii) sold, reengineered and sold or otherwise provided to any third party for any use or purpose, (iii) provided to any agent or other third party for any reason whatsoever, or (iv) used for diagnostic or therapeutic purposes. Acceptance of these terms and conditions shall be deemed by RECIPIENT upon delivery of BIOLOGICAL PRODUCT(S) from ArunA Biomedical. 
Product Use Agreement
BIOLOGICAL PRODUCT(S) purchased and DERIVATIVE BIOLOGICAL MATERIALS resulting from the use of ArunA Biomedical BIOLOGICAL PRODUCT(S) shall not be: (i) used for any purpose other than internal research and internal research services, (ii) sold, reengineered and sold or otherwise provided to any third party for any use or purpose, (iii) provided to any agent or other third party for any reason whatsoever, or (iv) used for diagnostic or therapeutic purposes. Acceptance of these terms and conditions shall be deemed by RECIPIENT upon delivery of BIOLOGICAL PRODUCT(S) from ArunA Biomedical. 

 

ArunA has entered into a technology licensing agreement with the University of Georgia Research Foundation (UGARF) that enables the company to commercialize adherent monolayer neural cell technology developed at the University.  The license allows the company exclusive worldwide rights to develop and commercialize neural cells derived from human embryonic stem cells.

ArunA has also entered into a research and commercialization license with the Wisconsin Alumni Research Foundation (WARF) for nonexclusive rights to their embryonic stem cell patents in research applications.

 

Patent US 6,200,806

Patent US 7,531,354,B2 

Patent US 8,178,089