Motor Neurons GFP+ HTScreening Kit

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Catalog: mMN7025HTS | US $998 | International $1,497

ArunA Biomedical's Mouse Motor Neurons are ideal for use in High-throughput fluorescent screening applications. Derived from transgenic mice expressing eGFP and using Hb9 motorneuron promoter enables easy tracking and visualization of spinal motor neurons without the need for additional fluorescent markers and extended cultures.

ArunA's GFP+ Mouse Motor Neurons express > 25% eGFP expressing mouse motor neurons differentiated from an embryonic stem cell line (HBG3) expressing eGFP under control of the HB9 promoter. A specific motor neuron maker, HB9 allows for easy identification and tracking of motor neuron cells.

Cell Type  Mouse motor neurons
Part Number:  7025
Species:  Mus musculus
Source:  HB9-GFP (HBG3) ESC
Morphology:  neural
Quantity:  7.5x105 cells per vial 

Shipping method:  dry ice
Storage:  liquid nitrogen
Growth Propertiesadherent, non-proliferative
Medium AB2 Basal Neural Medium and supplements

Pathogen testing:  Cells are tested for sterility and mycoplasma contamination.

Viability testing:  Post-cryostorage viability was determined to be approximately 75%. 

Kit format:

  • GFP+mMN™ Mouse Motor Neurons (Cat#mMN7025), 4 vials (~7.5 x 105 cells)
  • AB2 Basal Neural Media (Cat#7011.3) 1x500mL

Technical information:

Technical data sheet and cell culture protocols

 

Product Use Agreement

BIOLOGICAL PRODUCT(S) purchased from ArunA Biomedical shall not be: (i) used for any purpose other than internal research and internal research services, (ii) sold or otherwise provided to any third party for any use or purpose, or (iii) provided to any agent or other third party to provide services and or other services. Acceptance of BIOLOGICAL PRODUCT(S) from ArunA Biomedical shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires ArunA Biomedical prior written authorization. BIOLOGICAL PRODUCT(S) may not be used for diagnostic or therapeutic purposes.

 

In collaboration with Axion Biosystems, we have developed a protocol for use of ArunA's GFP+mMN Mouse Motor Neurons on Axion Biosystems' microelectrode array platform. The video below demostrates GFP+mMN Mouse Motor Neuron response when exposed to chemical antagonist.

Webinar: GFP+ Motor Neurons Development and in-vitro Functional Assessment on Microelectrode Arrays

 

 

Protocols for using GFP+mMN Mouse Motor Neurons on MEA

 

ArunA Biomedical's GFP+mMN Mouse Motor Neurons demonstrate spontaneous firing of action potentials on microelectrode array (Axion Biosystems). Image courtesy Raymond Swetenburg, University of Georgia.