Publications

Publications by Application:

Adenoviral Transduction
Amniotic Epithelial Cells
BoNT/A 
Cell Adhesion

Cell Based Assays
Cell Migration

Cell Morphology
Cell Proliferation
Cell-Cycle Alterations
Cellular Phenotyping
Cellular Reprogramming
Differentiation and Adhesion
Differentiation Capacity
Differentiation Markers
Disease Modeling

DNA Damage and Repair
DNA Methylation
Dopaminergic Differentiation
Electrophysiology
Epigenetic Regulation
Flow Cytometry
FoldingGenetic Modification

Germ Cell Isolation
Germ Cells
Glutamate (AMPA, Kainate)
Receptor Pharmacology

Glycan Expression
GPCR
High-Content Imaging
High-Throughput Screening
Human Neural Progenitor Cells
Human Lung Fibroblast
Immuno-Cytochemistry
Induced Pluripotent Stem Cells

in vivo Modeling
Ion Channel

Lectin Binding
Lentiviral Transduction
Mesenchymal Differentiation
Mesenchymal Progenitor Isolation
Micro RNA Regulation
Mitochondrial Gene Replacement
Membrane Proteins

Neural Cytotoxicity
Neural Development
Neural Differentiation
Neural Progenitor Isolation
Neural Proliferation
Neurite Outgrowth
Neurogenesis
Neuronal Differentiation
Neurotoxicity
Nucleofection
Oxidative Stress
Proliferation Rate
Proteomics
Quantitative PCR
RNA Sequencing
Splicing
Stem Cell Transplantation
Tissue Engineering
Toxicology Screening
Transcriptional Profiling
Transfection

Reviews by Application:

GPCR
High-Throughput Screening
Neural Differentiation
Synapse Formation

Presentations & Webinars:

Society of Toxicology 2012: Advancing the Science of Stem Cells in Toxicology

Conference Poster Presentations:

International Society for Stem Cell Research
Society of Neuroscience
Society of Toxicology

 


 

Publications and Posters

Adenoviral Transduction

References

ArunA Product

Adenoviral Transduction of Mesenchymal Stem Cells

Bosch P, Stice SL. Methods Mol Biol. 2007;407:265-74.

hMPro™

Back to Top


Amniotic Epithelial Cells

References ArunA Product

Generation of Human Induced Pluripotent Stem Cells from Amniotic Epithelial Cells

William L. Blake, Pfizer Corporation, et al ISSCR 2010.

(poster presentation)

viPS™
Vector Kit

Back to Top

BoNT/A

References ArunA Product

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

Cryopreserved Human Neuronal Cells Express SNAP-25 and Are Sensitive to BoNT/A

Coffield, Julie, et al. Interagency Botulism Coordinating Committee 2009.

(poster presentation)

hN2™

Back to Top

Cell Adhesion

References ArunA Product

Adhesion strength-based, label-free isolation of human pluripotent stem cells.

Singh A, Suri S, Lee T, Chilton JM, Cooke MT, Chen W, Fu J, Stice SL, Lu H, McDevitt TC, García AJ.

hNP1

hN2

Back to Top

Cell Based Assays

References ArunA Product

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

Development of a novel high throughput screening/high content imaging amenable assay for cell proliferation and migration in human embryonic stem cell derived neural progenitors

Powe, A et al. Society for Neuroscience 2011.

(poster presentation)

hNP1™

AB2™ ANS™

Development of a Novel HTS Amenable Assay Platform for Visual Assessment of Human Embryonic Stem Cell Derived Neural Progenitor Migration

Powe, A et al. ISSCR 2011.

(poster presentation)

hNP1™

AB2™ ANS™

A Novel HTS-Adaptable Assay for Cell Migration of hESC-Derived Progenitors

Powe, A et al. HH Workshop 2011.

(poster presentation)

hNP1™

AB2™ ANS™

Development of a Novel Assay Platform for Visual Assessment of hESC Derived Neural Progenitor Cell Migration in Response to Neurotoxicants

Powe, A, et al. SOT 2011.

(poster presentation)

hNP1™

AB2™ ANS™

Identification of Stimulators and Inhibitors of Cell Migration in Human Embryonic Stem Cell Derived Neural Progenitors Using a Novel, High Throughput Amenable Assay Platform

Powe, A, et al. ASCB 2010.

(poster presentation)

hNP1™

AB2™ ANS™

An SMA Project Report: Neural Cell-Based Assays Derived From Human Embryonic Stem Cells

Wilson PG, et al. Stem Cells Dev. 2007 Dec;16(6):1027-41.

hNP1™

AB2™ ANS™

Back to Top

 


Cell Migration

References ArunA Product

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

Development of a novel high throughput screening/high content imaging amenable assay for cell proliferation and migration in human embryonic stem cell derived neural progenitors

Powe, A et al. Society for Neuroscience 2011.

(poster presentation)

hNP1™

AB2™ ANS™

Development of a Novel HTS Amenable Assay Platform for Visual Assessment of Human Embryonic Stem Cell Derived Neural Progenitor Migration

Powe, A et al. ISSCR 2011.

(poster presentation)

hNP1™

AB2™ ANS™

A Novel HTS-Adaptable Assay for Cell Migration of hESC-Derived Progenitors

Powe, A et al. HH Workshop 2011.

(poster presentation)

hNP1™

AB2™ ANS™

Development of a Novel Assay Platform for Visual Assessment of hESC Derived Neural Progenitor Cell Migration in Response to Neurotoxicants

Powe, A, et al. SOT 2011.

(poster presentation)

hNP1™

AB2™ ANS™

Identification of Stimulators and Inhibitors of Cell Migration in Human Embryonic Stem Cell Derived Neural Progenitors Using a Novel, High Throughput Amenable Assay Platform

Powe, A, et al. ASCB 2010.

(poster presentation)

hNP1™

AB2™ ANS™

Effects of Topography on the Functional Development of Human Neural Progenitor Cell

Wu ZZ, et al. Biotechnol Bioeng. 2010 Jul 1;106(4):649-59.

hNP1™

AB2™ ANS™

Characterization of Micropatterned Nanofibrous Scaffolds for Neural Network Activity Readout for High-Throughput Screening

Wang L, Kisaalita WS. J Biomed Mater Res B Appl Biomater. 2010 Jul;94(1):238-49.

hNP1™

AB2™ ANS™

Human Neural Progenitors Express Functional Lysophospholipid Receptors that Regulate Cell Growth and Morphology

Hurst JH, et al. BMC Neurosci. 2008 Dec 11;9:118.

hNP1™

AB2™ ANS™

hN2™

Back to Top

 

Cell Morphology

References ArunA Product

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

Human Neural Progenitors Express Functional Lysophospholipid Receptors that Regulate Cell Growth and Morphology

Hurst JH, et al. BMC Neurosci. 2008 Dec 11;9:118.

hNP1™

AB2™ ANS™

hN2™

Back to Top

 


Cell Proliferation

References ArunA Product

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

Development of a novel high throughput screening/high content imaging amenable assay for cell proliferation and migration in human embryonic stem cell derived neural progenitors

Powe, A et al. Society for Neuroscience 2011.

(poster presentation)

hNP1™

AB2™ ANS™

Endothelium-Induced Proliferation and Electrophysiological Differentiation of Human Embryonic Stem Cell-Derived Neuronal Precursors

Lai B, et al. Stem Cells Dev. 2008 Jun;17(3):565-72.

hNP1™

AB2™ ANS™

Back to Top

 


Cell-Cycle Alterations

References ArunA Product

Consequences of Ionizing Radiation-Induced Damage in Human Neural Stem Cells

Acharya MM, et al. Free Radic Biol Med. 2010 Sep 6. [Epub ahead of print]

hNP1™

AB2™
ANS™

Back to Top

 

 

Cellular Phenotyping

References ArunA Product

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

KIT Ligand and Bone Morphogenetic Protein Signaling Enhances Human Embryonic Stem Cell to Germ-like Cell Differentiation

West FD, et al. Hum Reprod. 2010 Jan;25(1):168-78. Epub 2009 Oct 19.

hNP1™

AB2™ ANS™

Back to Top

 


Cellular Reprogramming

References ArunA Product

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

Proliferative, Adherent Neural Progenitors Derived from Human Induced Pluripotent Stem Cells

Chilton, JM, et al. SENN&GASNC 2010  

Conference Abstract, Published on Mar 2010 Front.
Neurosci.  doi:  10.3389/conf.fnins.2010.04.00030

(poster presentation)

hNP1™

AB2™ ANS™

viPS™ Vector Kit

hMPro™

High-Efficiency Induction of Neural Conversion in hESCs and hiPSCs with a Single Chemical Inhibitor of TGF-beta Superfamily Receptors

Zhou J, et al. Stem Cells. 2010 Aug 23. [Epub ahead of print]

viPS™ Vector Kit

Porcine Induced Pluripotent Stem Cells Produce Chimeric Offspring

West FD, et al. Stem Cells Dev. 2010 Aug;19(8):1211-20.

viPS™ Vector Ki

Proliferative, Adherent Neural and Mesenchymal Progenitors Derived from Human Induced Pluripotent Stem Cells

Chilton, JM et al. ISSCR 2010

(poster presentation)

viPS™ Vector Kit

hNP1™

AB2™ ANS™

hMPro™

Generation of Human Induced Pluripotent Stem Cells from Amniotic Epithelial Cells

William L. Blake, Pfizer Corporation, et al ISSCR 2010

(poster presentation)

viPS™ Vector Kit

Formation of Human Induced Pluripotent Stem Cells is Accelerated Using Six Reprogramming Factors

Chilton, Jm et al. ISSCR 2009

(poster presentation)

viPS™ Vector Kit

Back to Top

 

Differentiation and Adhesion

References ArunA Product

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

Exploring Cellular Adhesion and Differentiation in a Micro-/Nano-Hybrid Polymer Scaffold

Cheng K, Kisaalita WS. Biotechnol Prog. 2010 May-Jun;26(3):838-46.

hNP1™

AB2™
ANS™

Back to Top

 

Differentiation Capacity

References ArunA Product
 
Cristofanilli M, Cymring B, Lu A, Rosenthal H, Sadiq SA.
Multiple Sclerosis Research Center of New York, New York, NY 10019, USA.
 
Neuroscience. 2013 Oct 10;250:614-21. doi: 10.1016/j.neuroscience.2013.07.022. Epub 2013 Jul 19.
 
 

hNP1™

AB2™

ANS™

Derivation of sensory neurons and neural crests from human neural progenitors, hNP1

Guo X, Spradling S, Stancescu M, Lambert S, Hickman JJ. 
Biomaterials 2013 Mar 14.  [Epub ahead of print]

This study reports efficient differentiation of ArunA’s hNP1™ Human Neural Progenitor Cells into sensory neurons and Schwann cells  in an adherent culture system. Here, hNP1™ cells, were differentiated into neural crest cells which stained positive for neural crest markers HNK1 (90%) and P75 (99%). These neural crest cells were further differentiated, peripherin and cGluT1 positive sensory neurons, 51.3% of the cells in culture. 23 of the 24 recorded sensory neuron cells were capable of firing action potentials (APs). The remaining ~48.0% of the cells were identified as S100 positive Schwann cells. These cells are ideal for multiple applications including drug and toxicology screening.

 hNP1TM

AB2TM

ANSTM 

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

Microvascular Mural cell functionality of human embryonic stem cell derived mesenchymal cells

Boyd NL, et al. Tissue Eng Part A. 2011 Feb 1. [Epub ahead of print]

hMPro™

Lineage-specific promoter DNA methylation patterns segregate adult progenitor cell types

Sørensen AL, et al. Stem Cells Dev. 2010 Aug;19(8):1257-66.

hMPro™

Back to Top


Differentiation Markers

References ArunA Product

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

L1TD1 is a marker for undifferentiated human embryonic stem cells

Wong RC, et al. PLoS One. 2011 April 29; (6)4:e19355.                                        

hNP1™

AB2™
ANS™

Back to Top

Disease Modeling

References

ArunA Product

Cerebrospinal fluid derived from progressive multiple sclerosis patients promotes neuronal and oligodendroglial differentiation of human neural precursor cells in vitro

Cristofanilli M1, Cymring B, Lu A, Rosenthal H, Sadiq SA

hNP1

Back to Top

DNA Damage and Repair

References ArunA Product

Consequences of ionizing radiation-induced damage in human neural stem cells

Acharya MM, et al. Free Radic Biol Med. 2010 Sep 6. [Epub ahead of print]

hNP1™

AB2™
ANS™

Back to Top

 

DNA Methylation

References ArunA Product

Lineage-specific promoter DNA methylation patterns segregate adult progenitor cell types

Sørensen AL, et al. Stem Cells Dev. 2010 Aug;19(8):1257-66.

hMPro™

Back to Top

 

Dopaminergic Differentiation

References ArunA Product

Stage-specific role for shh in dopaminergic differentiation of human embryonic stem cells induced by stromal cells

Swistowska AM, et al. Stem Cells Dev. 2010 Jan;19(1):71-82.

hNP1™

AB2™ ANS™

Glial cell line-derived neurotrophic factor enhances in vitro differentiation of mid-/hindbrain neural progenitor cells to dopaminergic-like neurons

Young A, et al. J Neurosci Res. 2010 Nov 15;88(15):3222-32.

hNP1™

AB2™ ANS™

Back to Top

 

Electrophysiology

References ArunA Product
 
This study reports efficient differentiation of  ArunA’s hNP1™ Human Neural Progenitor Cells into sensory neurons and Schwann cells  in an adherent culture system. Here, hNP1™ cells, were differentiated into neural crest cells which stained positive for neural crest markers HNK1 (90%) and P75 (99%). These neural crest cells were further differentiated, peripherin and cGluT1 positive sensory neurons, 51.3% of the cells in culture. 23 of the 24 recorded sensory neuron cells were capable of firing action potentials (APs). The remaining ~48.0% of the cells were identified as S100 positive Schwann cells. These cells are ideal for multiple applications including drug and toxicology screening.

Guo XSpradling SStancescu MLambert SHickman JJ

http://www.ncbi.nlm.nih.gov/pubmed/23498896

hNP1™

Characterization of Embryonic Stem Cell-derived Neurons on High-throughput Automated Patch Clamp System

Jiang, X., et al. ISSCR 2011.

(poster presentation)
 

hNP1™

AB2™ ANS™

hN2™

Endothelium-induced proliferation and electrophysiological differentiation of human embryonic stem cell-derived neuronal precursors

Lai B, et al. Stem Cells Dev. 2008 Jun;17(3):565-72.

hNP1™

AB2™ ANS™

Differentiation of Human Embryonic Stem Cell Derived Neural Progenitors into Functionally Responsive Populations in the Absence of Exogenous EGF

D.W. Machacek, et al. ISSCR  2008

(poster presentation)

hN2™

Back to Top

 

Epigenetic Regulation

References ArunA Product

Lineage-specific promoter DNA methylation patterns segregate adult progenitor cell types

Sørensen AL, et al. Stem Cells Dev. 2010 Aug;19(8):1257-66.

hMPro™

Back to Top

 

Flow Cytometry

References ArunA Product

KIT ligand and bone morphogenetic protein signaling enhances human embryonic stem cell to germ-like cell differentiation

West FD, et al. Hum Reprod. 2010 Jan;25(1):168-78. Epub 2009 Oct 19.

hNP1™

AB2™ ANS™

Differing Lectin Binding Profiles among Human Embryonic Stem Cells and Derivatives Aid in the Isolation of Neural Progenitor Cells

Dodla, Mahesh C. et. al. 2011 Aug 5. PLoS ONE 6(8): e23266. doi:10.1371/journal.pone.0023266

hNP1™

hMPro™

AB2™ ANS™

Back to Top

 

Folding

References ArunA Product

Gene processing control loops suggested by sequencing, splicing, and RNA folding.

Jeffries CD, et al. BMC Bioinformatics. 2010 Dec 20;11:602.

hNP1™

AB2™
ANS™

Back to Top

 

Genetic Modification

References ArunA Product

Nonviral Gene Delivery in Neural Progenitors Derived from Human Pluripotent Stem Cells

Dhara, Sujoy K. et. al. Methods in Mol Biology, 2011, Vol. 767, Part 5, 343-354, doi: 10.1007/978-1-61779-201-4_2

hNP1™

AB2™  ANS™

Proliferative, adherent neural progenitors derived from human induced pluripotent stem cells

Chilton, JM, et al. SENN&GASNC 2010  

Conference Abstract, Published on Mar 2010 Front.
Neurosci.  doi:  10.3389/conf.fnins.2010.04.00030

(poster presentation)

hNP1™

AB2™  ANS™

viPS™ Vector Kit

Genetic manipulation of neural progenitors derived from human embryonic stem cells

Dhara SK, et al. Tissue Eng Part A. 2009 Nov;15(11):3621-34.

hNP1™

AB2™
ANS™

Adenoviral transduction of mesenchymal stem cells

Bosch P, Stice SL. Methods Mol Biol. 2007;407:265-74.

hMPro™

Formation of Human Induced Pluripotent Stem Cells is Accelerated Using Six Reprogramming Factors

Chilton, Jm et al. ISSCR 2009

(poster presentation)

viPS™ Vector Kit

Back to Top

 

Germ Cell Isolation

References ArunA Product

Porcine induced pluripotent stem cells produce chimeric offspring

West FD, et al. Stem Cells Dev. 2010 Aug;19(8):1211-20.

viPS™
Vector Kit

Back to Top

 

Germ Cells

References ArunA Product

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

Porcine induced pluripotent stem cells produce chimeric offspring

West FD, et al. Stem Cells Dev. 2010 Aug;19(8):1211-20.

viPS™
Vector Kit

Back to Top

 

Glutamate (AMPA, Kainate) Receptor Pharmacology

References ArunA Product

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

High-throughput screening in embryonic stem cell-derived neurons identifies potentiators of alpha-amino-3-hydroxyl-5-methyl-4-isoxazolepropionate-type glutamate receptors

Summary: Mouse vs Human neural cells produce different results in drug discovery assay. Mouse ESC and ArunA neural cells derived from human ESC were used in a FLIPR high throughput assay for AMPA potentiation compound testing.  Both cells generated repeatable and robust cells however results did differ.  Table 1 summarizes the results between the two species.  The authors conclude ….”recent advances suggest that hES cell-derived neurons can be reproducibly scaled and quality-controlled during differentiation to consider moving directly to primary HTS in human cells …. this may eliminate translational differences between murine and human cellular generated data, as was observed in this study where 4/21 hits showed a 10 fold affinity difference between mouse and human cells.”

McNeish J, et al. J Biol Chem. 2010 May 28;285(22):17209-17.

hNP1™

AB2™ ANS™

Back to Top

 

Glycan Expression

References ArunA Product
Differing Lectin Binding Profiles among Human Embryonic Stem Cells and Derivatives Aid in the Isolation of Neural Progenitor Cells

Dodla, Mahesh C. et. al.
PLoS ONE. 2011 Aug 5. 6(8): e23266. doi:10.1371/journal.pone.0023266

hNP1™

hMPro™

AB2™ ANS™

Back to Top

 

 

GPCR

References ArunA Product

Differentiation of Human Embryonic Stem Cell Derived Neural Progenitors into Functionally Responsive Populations in the Absence of Exogenous EGF

D.W. Machacek, et al. ISSCR  2008

(poster presentation)

hN2™

G-protein Coupled Receptor Expression Patterns Are Altered as Human Embryonic Stem Cell Derived Neural Cultures Differentiate

D. W. Machacek, et al. Society for Neuroscience 2008

(poster presentation)

hN2™

Back to Top

 

 

High-Content Imaging

References ArunA Product

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

High Content Image Analysis of Neurogenesis Using Human Embryonic Stem Cell Derived Neural Cells

Sirenko, O et al. Society of Toxicology 2012.

(poster presentation)

hNP1™

hN2™

AB2™ ANS™

Development of a novel high throughput screening/high content imaging amenable assay for cell proliferation and migration in human embryonic stem cell derived neural progenitors

Powe, A et al. Society for Neuroscience 2011.

(poster presentation)

hNP1™

AB2™ ANS™

Quantitative assessment of neurite outgrowth in human embryonic stem cell-derived hN2 cells using automated high-content image analysis

Summary: Thaw and use neuronal cells derived from hESC generates rapid and repeatable results in a neurite outgrowth high content imaging toxicity assay. ArunA hN2 human embryonic stem cell derived neural cells were used in an automated high-content image analysis to measure neurite outgrowth in vitro. These thaw and use hN2 cells generated neurites within six hrs and by 24  and 48 hrs there was a concentration-dependent decrease in neurite outgrowth and ATP-content were observed following treatment with either bisindolylmaleimide I, U0126, lithium chloride, sodium orthovanadate and brefeldin A  (Figure 6).  All of which have previously been shown to inhibit neurite outgrowth in primary rodent neural cultures.  The authors suggest that this study “supports the hypothesis that measurements of neurite outgrowth are more sensitive than measures of cell health in the context of neurotoxicity screening… and  hN2 cells provide a model in which to investigate the processes of neurite outgrowth in the context of human biology.

Harrill JA, et al. Neurotoxicology. 2010 Jun;31(3):277-90.

hN2™

Comparative sensitivity of human and rat neural cultures to chemical-induced inhibition of neurite outgrowth

Summary: In an extension of the above study the ArunA hN2™ cells demonstrated that human neural cultures were more sensitive to neurite outgrowth inhibitors than primary rat cortical neuron cultures automated high-content image analysis to measure neurite outgrowth in vitro.

Harrill JA, et al. 2011. Toxico Appl Pharmacol. Epub 2011.

hN2™

Back to Top

 

High-Throughput Screening

References ArunA Product

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

Development of a novel high throughput screening/high content imaging amenable assay for cell proliferation and migration in human embryonic stem cell derived neural progenitors

Powe, A et al. Society for Neuroscience 2011.

(poster presentation)

hNP1™

AB2™ ANS™

Quantitative assessment of neurite outgrowth in human embryonic stem cell-derived hN2 cells using automated high-content image analysis

Summary: Thaw and use neuronal cells derived from hESC generates rapid and repeatable results in a neurite outgrowth high content imaging toxicity assay. ArunA hN2 human embryonic stem cell derived neural cells were used in an automated high-content image analysis to measure neurite outgrowth in vitro. These thaw and use hN2 cells generated neurites within six hrs and by 24  and 48 hrs there was a concentration-dependent decrease in neurite outgrowth and ATP-content were observed following treatment with either bisindolylmaleimide I, U0126, lithium chloride, sodium orthovanadate and brefeldin A  (Figure 6).  All of which have previously been shown to inhibit neurite outgrowth in primary rodent neural cultures.  The authors suggest that this study “supports the hypothesis that measurements of neurite outgrowth are more sensitive than measures of cell health in the context of neurotoxicity screening… and  hN2 cells provide a model in which to investigate the processes of neurite outgrowth in the context of human biology.

Harrill JA, et al. Neurotoxicology. 2010 Jun;31(3):277-90.

hN2™

High-throughput screening in embryonic stem cell-derived neurons identifies potentiators of alpha-amino-3-hydroxyl-5-methyl-4-isoxazolepropionate-type glutamate receptors

Summary: Mouse vs Human neural cells produce different results in drug discovery assay. Mouse ESC and ArunA neural cells derived from human ESC were used in a FLIPR high throughput assay for AMPA potentiation compound testing.  Both cells generated repeatable and robust cells however results did differ.  Table 1 summarizes the results between the two species.  The authors conclude ….”recent advances suggest that hES cell-derived neurons can be reproducibly scaled and quality-controlled during differentiation to consider moving directly to primary HTS in human cells …. this may eliminate translational differences between murine and human cellular generated data, as was observed in this study where 4/21 hits showed a 10 fold affinity difference between mouse and human cells.”

McNeish J, et al. J Biol Chem. 2010 May 28;285(22):17209-17.

hNP1™

AB2™ ANS™

Back to Top

 

Human Neural Progenitor Cells

References ArunA Product

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

High-Efficiency Induction of Neural Conversion in hESCs and hiPSCs with a Single Chemical Inhibitor of TGF-beta Superfamily Receptors

Zhou J, et al. Stem Cells. 2010 Aug 23. [Epub ahead of print]

viPS™ Vector Kit

Back to Top

 

Human Lung Fibroblast

References ArunA Product

High-Efficiency Induction of Neural Conversion in hESCs and hiPSCs with a Single Chemical Inhibitor of TGF-beta Superfamily Receptors

Zhou J, et al. Stem Cells. 2010 Aug 23. [Epub ahead of print]

viPS™ Vector Kit

Proliferative, Adherent Neural and Mesenchymal Progenitors Derived from Human Induced Pluripotent Stem Cells

Chilton, JM et al. ISSCR 2010

(poster presentation)

NP1™

AB2™
ANS™

viPS™ Vector Kit

hMPro™

Formation of Human Induced Pluripotent Stem Cells is Accelerated Using Six Reprogramming Factors

Chilton, Jm et al. ISSCR 2009

(poster presentation)

viPS™ Vector Kit

Back to Top

 

Immuno-cytochemistry

References ArunA Product
Differing Lectin Binding Profiles among Human Embryonic Stem Cells and Derivatives Aid in the Isolation of Neural Progenitor Cells

Dodla, Mahesh C. et. al. 2011 Aug 5. PLoS ONE 6(8): e23266. doi:10.1371/journal.pone.0023266

hNP1™

hMPro™

AB2™ ANS™

Back to Top

 

Induced Pluripotent Stem Cells

References ArunA Product

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

Proliferative, Adherent Neural Progenitors Derived from Human Induced Pluripotent Stem Cells

Chilton, JM, et al. SENN&GASNC 2010  

Conference Abstract, Published on Mar 2010 Front.
Neurosci.  doi:  10.3389/conf.fnins.2010.04.00030

(poster presentation)

hNP1™

AB2™ ANS™

viPS™ Vector Kit

hMPro™

Human Induced Pluripotent Stem Cells Differentiate Into Osteo- And Chondrogenic Progenitors

Chilton, JM et al. ISSCR 2011

(poster presentation)

viPS™ Vector Kit

hMPro™

Osteo/Chondrogenic Progenitors Derived from Human Induced Pluripotent Stem Cells

Chilton, JM et al. HH Workshop 2011

(poster presentation)

viPS™ Vector Kit

hMPro™

High-Efficiency Induction of Neural Conversion in hESCs and hiPSCs with a Single Chemical Inhibitor of TGF-beta Superfamily Receptors

Zhou J, et al. Stem Cells. 2010 Aug 23. [Epub ahead of print]

viPS™ Vector Kit

Porcine Induced Pluripotent Stem Cells Produce Chimeric Offspring

West FD, et al. Stem Cells Dev. 2010 Aug;19(8):1211-20.

viPS™ Vector Kit

Proliferative, Adherent Neural and Mesenchymal Progenitors Derived from Human Induced Pluripotent Stem Cells

Chilton, JM et al. ISSCR 2010

(poster presentation)

viPS™ Vector Kit

hNP1™

AB2™ ANS™

hMPro™

Generation of Human Induced Pluripotent Stem Cells from Amniotic Epithelial Cells

William L. Blake, Pfizer Corporation, et al ISSCR 2010

(poster presentation)

viPS™ Vector Kit

Formation of Human Induced Pluripotent Stem Cells is Accelerated Using Six Reprogramming Factors

Chilton, Jm et al. ISSCR 2009

(poster presentation)

viPS™ Vector Kit

Back to Top

 

in vivo Modeling

References ArunA Product

Effect of human neural precursor cell transplantation on endogenous neurogenesis after focal cerebral ischemia in the rat.

Jin K, et al. Brain Res. 2011 Feb 16; 1374:56-52. Epub 2010 Dec 16.

hNP1™

AB2™ ANS™

Delayed transplantation of human neural precursor cells improves outcome from focal cerebral ischemia in aged rats.

Jin K, et al. Aging Cell. 2010 Dec;9(6): 1076-83.

hNP1™

AB2™ ANS™

Transplantation of human neural precursor cells in Matrigel scaffolding improves outcome from focal cerebral ischemia after delayed postischemic treatment in rats.

Jin K, et al. J Cereb Blood Flow Metab. 2010 Mar;30(3):534-44. Epub 2009 Oct 14.

hNP1™ AB2™ ANS™

Back to Top

 

Ion Channel

References ArunA Product

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

Ion Channel Expression Patterns and Functional Response in Human Embryonic Stem Cell Derived Cell Lines

D. W. Machacek, et al. Society for Neuroscience 2008

(poster presentation)

hN2™

Ion channels and ionotrophic receptors in human embryonic stem cell derived neural progenitors

Summary: In this study ArunA neural progenitor (hNP1™) cells and cells differentiated from the hNP1™ cells were characterized under non directed basal conditions where bFGF was removed from the culture medium.  Using these same basal (low cost) conditions ArunA hNP1™ cells under whole-cell voltage clamp exhibited delayed rectifier potassium channel currents and exhibited tetrodotoxin sensitive, voltage-dependent sodium channel current.  Although previous studies have demonstrated functional electrophysiological properties when hESC cells are differentiated to neural cells these prior studies used expensive cocktails of growth factors in extensive cultures.  ArunA hNP1™ cells can be used in higher throughput electrophysiological studies using a minimal cost format. 

Young A, et al. Neuroscience.  2011 Sep 29;192:793-805. Epub 2011 Jun 7.

hNP1™

AB2™  ANS™

Back to Top

 

Lectin Binding

References ArunA Product
Differing Lectin Binding Profiles among Human Embryonic Stem Cells and Derivatives Aid in the Isolation of Neural Progenitor Cells

Dodla, Mahesh C. et. al.
PLoS ONE. 2011 Aug 5. 6(8): e23266. doi:10.1371/journal.pone.0023266

hNP1™

hMPro™

AB2™ ANS™

Back to Top


Lentiviral Transduction

References ArunA Product

Genetic Manipulation of Neural Progenitors Derived from Human Embryonic Stem Cells

Dhara SK, et al. Tissue Eng Part A. 2009 Nov;15(11):3621-34.

hNP1™

 

AB2™
ANS™

Formation of Human Induced Pluripotent Stem Cells is Accelerated Using Six Reprogramming Factors

Chilton, Jm et al. ISSCR 2009

(poster presentation)

viPS™ Vector Kit

Proliferative, Adherent Neural Progenitors Derived from Human Induced Pluripotent Stem Cells

Chilton, JM, et al. SENN&GASNC 2010  

Conference Abstract, Published on Mar 2010 Front.
Neurosci.  doi:  10.3389/conf.fnins.2010.04.00030

(poster presentation)

hNP1™

AB2™  ANS™

viPS™ Vector Kit

Back to Top

 

Mesenchymal Differentiation

References ArunA Product

Human Induced Pluripotent Stem Cells Differentiate Into Osteo- And Chondrogenic Progenitors

Chilton, JM et al. ISSCR 2011

(poster presentation)

viPS™ Vector Kit

hMPro™

Osteo/Chondrogenic Progenitors Derived from Human Induced Pluripotent Stem Cells

Chilton, JM et al. HH Workshop 2011

(poster presentation)

viPS™ Vector Kit

hMPro™

Back to Top

 

Mesenchymal Progenitor Isolation

References ArunA Product

Human embryonic stem cell-derived mesoderm-like epithelium transitions to mesenchymal progenitor cells

Boyd NL, et al. Tissue Eng Part A. 2009 Aug;15(8):1897-907.

hMPro™

Back to Top

 

Micro RNA Regulation

References ArunA Product

Nuclear and cytoplasmic localization of neural stem cell microRNAs

Jeffries CD, et al. RNA. 2011 Apr 17;(4): 675-86. Epub 2011 Mar 1.

hNP1™

AB2™
ANS™

Back to Top

 

Mitochondrial Gene Replacement

References ArunA Product

Mitochondrial Gene Replacement in Human Pluripotent Stem Cell-Derived Neural Progenitors 

Iyer, S. et. al. Gene Therapy. 15 September 2011. doi:10.1038/gt.2011.134

hNP1™

AB2™
ANS™

Back to Top

 

Membrane Proteins

References ArunA Product

Membrane proteomic signatures of karyotypically normal and abnormal human embryonic stem cell  lines and derivatives

Gerwe, B et al. Proteomics, 2011 Apr 12 [Epub ahead of print]

hNP1™

hMPro™

Back to Top

 

Neural Cytotoxicty

References ArunA Product

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

High Content Image Analysis of Neurogenesis Using Human Embryonic Stem Cell Derived Neural Cells

Sirenko, O et al. Society of Toxicology 2012.

(poster presentation)

hNP1™

hN2™

AB2™
ANS™

Back to Top

 

Neural Development

References ArunA Product

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

Mitochondrial Gene Replacement in Human Pluripotent Stem Cell-Derived Neural Progenitors 

Iyer, S. et. al. Gene Therapy. 15 September 2011. doi:10.1038/gt.2011.134

hNP1™

AB2™
ANS™

Back to Top


Neural Differentiation

References ArunA Product

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

High Content Analysis of Neural Stem Cell Expansion and Differentiation

Sirenko, O et al. Society for Neuroscience 2011.

(poster presentation)

hNP1™

AB2™ ANS™

Nonviral Gene Delivery in Neural Progenitors Derived from Human Pluripotent Stem Cells

Dhara, Sujoy K. et. al. Methods in Mol Biology, 2011, Vol. 767, Part 5, 343-354, doi: 10.1007/978-1-61779-201-4_2

hNP1™

AB2™ ANS™

Differing Lectin Binding Profiles among Human Embryonic Stem Cells and Derivatives Aid in the Isolation of Neural Progenitor Cells

Dodla, Mahesh C. et. al.
PLoS ONE. 2011 Aug 5. 6(8): e23266. doi:10.1371/journal.pone.0023266

hNP1™

hMPro™

AB2™ ANS™

High Content Analysis of Neural Stem Cell Expansion and Differentiation

Sirenko, O et al. ISSCR 2011

(poster presentation)

hNP1™

AB2™ ANS™
 

Ion channels and ionotrophic receptors in human embryonic stem cell derived neural progenitors

Summary: In this study ArunA neural progenitor (hNP1™) cells and cells differentiated from the hNP1™ cells were characterized under non directed basal conditions where bFGF was removed from the culture medium.  Using these same basal (low cost) conditions ArunA hNP1™ cells under whole-cell voltage clamp exhibited delayed rectifier potassium channel currents and exhibited tetrodotoxin sensitive, voltage-dependent sodium channel current.  Although previous studies have demonstrated functional electrophysiological properties when hESC cells are differentiated to neural cells these prior studies used expensive cocktails of growth factors in extensive cultures.  ArunA hNP1™ cells can be used in higher throughput electrophysiological studies using a minimal cost format. 

Young A, et al. Neuroscience.  2011 Sep 29;192:793-805. Epub 2011 Jun 7.

hNP1™

AB2™ ANS™

Proliferative, adherent neural progenitors derived from human induced pluripotent stem cells

Chilton, JM, et al. SENN&GASNC 2010

Conference Abstract, Published on Mar 2010 Front.
Neurosci.  doi:  10.3389/conf.fnins.2010.04.00030

(poster presentation)

hNP1™

AB2™ ANS™

viPS™ Vector Kit

Neural differentiation of human embryonic stem cells at the ultrastructural level

Mumaw JL, et al. Microsc Microanal. 2010 Feb;16(1):80-90. Review.

hNP1™

 

AB2™ ANS™

Stage-specific role for shh in dopaminergic differentiation of human embryonic stem cells induced by stromal cells

Swistowska AM, et al. Stem Cells Dev. 2010 Jan;19(1):71-82.

hNP1™

 

AB2™ ANS™

Highly efficient differentiation and enrichment of spinal motor neurons derived from human and monkey embryonic stem cells

Wada T, et al. PLoS One. 2009 Aug 24;4(8):e6722.

hNP1™

 

AB2™ ANS™

Endothelium-induced proliferation and electrophysiological differentiation of human embryonic stem cell-derived neuronal precursors

Lai B, et al. Stem Cells Dev. 2008 Jun;17(3):565-72.

hNP1™

 

AB2™ ANS™

Differentiation of Human Embryonic Stem Cell Derived Neural Progenitors into Functionally Responsive Populations in the Absence of Exogenous EGF

D.W. Machacek, et al. ISSCR  2008

(poster presentation)

hNP1™

 

Development of Neural Cultures From Human Embryonic Stem Cell Derived Neural Progenitors

D. W. Machacek, et al. Society for Neuroscience 2007

(poster presentation)

hNP1™

 

High-Efficiency Induction of Neural Conversion in hESCs and hiPSCs with a Single Chemical Inhibitor of TGF-beta Superfamily Receptors

Zhou J, et al. Stem Cells. 2010 Aug 23. [Epub ahead of print]

viPS™ Vector Kit

Back to Top

 

Neural Progenitor Isolation

References ArunA Product

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

Human neural progenitor cells derived from embryonic stem cells in feeder-free cultures

Dhara SK, et al. Differentiation. 2008 May;76(5):454-64.

hNP1™

 

AB2™ ANS™

Long-term proliferation of human embryonic stem cell-derived neuroepithelial cells using defined adherent culture conditions

Shin S, et al. Stem Cells. 2006 Jan;24(1):125-38.

hNP1™

AB2™ ANS™

Back to Top

 

Neural Proliferation

References ArunA Product

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

 

Spirulina promotes stem cell genesis and protects against LPS induced declines in neural stem cell proliferation

Bachstetter AD, et. al. PLoS. 2010 May 5; 5(5):e10496

hNP1™

AB2™ ANS™

High Content Analysis of Neural Stem Cell Expansion and Differentiation

Sirenko, O et al. Society for Neuroscience 2011.

(poster presentation)

hNP1™

AB2™ ANS™

Back to Top

 

Neurite Outgrowth

References ArunA Product

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

High Content Image Analysis of Neurogenesis Using Human Embryonic Stem Cell Derived Neural Cells

Sirenko, O et al. Society of Toxicology 2012.

(poster presentation)

hNP1™

hN2™

AB2™
ANS™

Quantitative assessment of neurite outgrowth in human embryonic stem cell-derived hN2 cells using automated high-content image analysis

Summary: Thaw and use neuronal cells derived from hESC generates rapid and repeatable results in a neurite outgrowth high content imaging toxicity assay. ArunA hN2 human embryonic stem cell derived neural cells were used in an automated high-content image analysis to measure neurite outgrowth in vitro. These thaw and use hN2 cells generated neurites within six hrs and by 24  and 48 hrs there was a concentration-dependent decrease in neurite outgrowth and ATP-content were observed following treatment with either bisindolylmaleimide I, U0126, lithium chloride, sodium orthovanadate and brefeldin A  (Figure 6).  All of which have previously been shown to inhibit neurite outgrowth in primary rodent neural cultures.  The authors suggest that this study “supports the hypothesis that measurements of neurite outgrowth are more sensitive than measures of cell health in the context of neurotoxicity screening… and  hN2 cells provide a model in which to investigate the processes of neurite outgrowth in the context of human biology.

Harrill JA, et al. Neurotoxicology. 2010 Jun;31(3):277-90.

hN2™

Comparative sensitivity of human and rat neural cultures to chemical-induced inhibition of neurite outgrowth

Summary: In an extension of the above study the ArunA hN2™ cells demonstrated that human neural cultures were more sensitive to neurite outgrowth inhibitors than primary rat cortical neuron cultures automated high-content image analysis to measure neurite outgrowth in vitro.

Harrill JA, et al. 2011. Toxico Appl Pharmacol. Epub 2011.

hN2™

Back to Top

 

Neurogenesis

References ArunA Product

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

High Content Image Analysis of Neurogenesis Using Human Embryonic Stem Cell Derived Neural Cells

Sirenko, O et al. Society of Toxicology 2012.

(poster presentation)

hNP1™

hN2™

AB2™
ANS™

Spirulina promotes stem cell genesis and protects against LPS induced declines in neural stem cell proliferation

Bachstetter AD, et. al. PLoS. 2010 May 5; 5(5):e10496

hNP1™

AB2™ ANS™

Back to Top


Neuronal Differentiation

References ArunA Product

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

Mitochondrial Gene Replacement in Human Pluripotent Stem Cell-Derived Neural Progenitors 

Iyer, S. et. al. Gene Therapy. 15 September 2011. doi:10.1038/gt.2011.134

hNP1™

AB2™
ANS™

Effects of topography on the functional development of human neural progenitor cells

Wu ZZ, et al. Biotechnol Bioeng. 2010 Jul 1;106(4):649-59.

hNP1™

AB2™
ANS™

 

High Content Analysis of Neural Stem Cell Expansion and Differentiation

Sirenko, O et al. Society for Neuroscience 2011.

(poster presentation)

hNP1™

AB2™
ANS™

Back to Top


Neurotoxicity

References ArunA Product

Toxicogenomic Studies of Human Neural Cells Following Exposure to Organophosphorus Chemical Warfare Nerve Agent VX 
 

Gao, Xiugong, Ray Prabhati, et al. Neurochemical Research, Feb 2013 doi: 10.1007/s11064-013-0996-1

 

hN2TM

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

Distinct generation, pharmacology, and distribution of sphingosine 1-phosphate and dihydrosphingosine 1-phosphate in human neural progenitor cells.

Callihan P, et al. Neuropharmacology. 2011 Oct 15. [Epub ahead of print] 

hNP1™

AB2™
ANS™

Comparative sensitivity of human and rat neural cultures to chemical-induced inhibition of neurite outgrowth

Summary: In an extension of the above study the ArunA hN2™ cells demonstrated that human neural cultures were more sensitive to neurite outgrowth inhibitors than primary rat cortical neuron cultures automated high-content image analysis to measure neurite outgrowth in vitro.

Harrill JA, et al. 2011. Toxico Appl Pharmacol. Epub 2011.

hN2™

Cryopreserved Human Neuronal Cells Express SNAP-25 and Are Sensitive to BoNT/A

Coffield, Julie, et al. Interagency Botulism Coordinating Committee 2009.

(poster presentation)

hN2™

High Content Analysis of Neural Stem Cell Expansion and Differentiation

Sirenko, O et al. Society for Neuroscience 2011.

(poster presentation)

hNP1™

AB2™ ANS™

Back to Top

 

Nucleofection

References ArunA Product

Nonviral Gene Delivery in Neural Progenitors Derived from Human Pluripotent Stem Cells

Dhara, Sujoy K. et. al. Methods in Mol Biology, 2011, Vol. 767, Part 5, 343-354, doi: 10.1007/978-1-61779-201-4_2

hNP1™

AB2™ ANS™

Back to Top

 

Oxidative Stress

References ArunA Product

Consequences of ionizing radiation-induced damage in human neural stem cells

Acharya MM, et al. Free Radic Biol Med. 2010 Sep 6. [Epub ahead of print]

hNP1™

AB2™
ANS™

Back to Top

 

Proliferation Rate

References ArunA Product

Exploring cellular adhesion and differentiation in a micro-/nano-hybrid polymer scaffold

Cheng K, Kisaalita WS. Biotechnol Prog. 2010 May-Jun;26(3):838-46.

hNP1™

AB2™
ANS™

Back to Top

 

Proteomics

References ArunA Product

Membrane proteomic signatures of karyotypically normal and abnormal human embryonic stem cell  lines and derivatives

Gerwe, B et. al. Proteomics, 2011 Apr 12 [Epub ahead of print]

hNP1™

AB2™ ANS™

hMPro™

Back to Top

 

Quantitative PCR

References ArunA Product

L1TD1 is a marker for undifferentiated human embryonic stem cells

Wong RC, et al. PLoS One. 2011 April 29; (6)4:e19355.

hNP1™

AB2™
ANS™

Back to Top

 

RNA Sequencing

References ArunA Product

Gene processing control loops suggested by sequencing, splicing, and RNA folding.

Jeffries CD, et al. BMC Bioinformatics. 2010 Dec 20;11:602.

hNP1™

AB2™
ANS™

Back to Top

 

Splicing

References ArunA Product

Gene processing control loops suggested by sequencing, splicing, and RNA folding.

Jeffries CD, et al. BMC Bioinformatics. 2010 Dec 20;11:602.

hNP1™

AB2™
ANS™

Back to Top

 


Stem Cell Transplantation

References ArunA Product
 
Chang DJ, Oh SH, Lee N, Choi C, Jeon I, Kim HS, Shin DA, Lee SE, Kim D, Song J.
 
*ArunA Biomedical is the exclusive manufacturer of ENStem-A Human Neural Progenitor Cells and ENStem-A is a co-brand label between ArunA Biomedical and sold through EMD Millipore Corporation.  
 
ArunA Biomedical manufactures and markets ENStem-A under the brand name, hNP1™

hNP1™

AB2™

ANS™

Transplantation of human neural precursor cells in Matrigel scaffolding improves outcome from focal cerebral ischemia after delayed postischemic treatment in rats

Jin K, et al. J Cereb Blood Flow Metab. 2010 Mar;30(3):534-44. Epub 2009 Oct 14.

hNP1™

 

AB2™ ANS™

Effect of human neural precursor cell transplantation on endogenous neurogenesis after focal cerebral ischemia in the rat

Jin K, et al. Brain Res. 2011 Feb 16; 1374:56-52. Epub 2010 Dec 16.

hNP1™

 

AB2™ ANS™

Delayed transplantation of human neural precursor cells improves outcome from focal cerebral ischemia in aged rats

Jin K, et al. Aging Cell. 2010 Dec;9(6): 1076-83.

hNP1™

 

AB2™ ANS™

Back to Top

 

Tissue Engineering

References ArunA Product

Characterization of micropatterned nanofibrous scaffolds for neural network activity readout for high-throughput screening

Wang L, Kisaalita WS. J Biomed Mater Res B Appl Biomater. 2010 Jul;94(1):238-49.

hNP1™

AB2™ ANS™

Exploring cellular adhesion and differentiation in a micro-/nano-hybrid polymer scaffold

Cheng K, Kisaalita WS. Biotechnol Prog. 2010 May-Jun;26(3):838-46.

hNP1™

AB2™ ANS™

Back to Top

 

Toxicology Screening

References ArunA Product

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

Quantitative assessment of neurite outgrowth in human embryonic stem cell-derived hN2 cells using automated high-content image analysis

Summary: Thaw and use neuronal cells derived from hESC generates rapid and repeatable results in a neurite outgrowth high content imaging toxicity assay. ArunA hN2 human embryonic stem cell derived neural cells were used in an automated high-content image analysis to measure neurite outgrowth in vitro. These thaw and use hN2 cells generated neurites within six hrs and by 24  and 48 hrs there was a concentration-dependent decrease in neurite outgrowth and ATP-content were observed following treatment with either bisindolylmaleimide I, U0126, lithium chloride, sodium orthovanadate and brefeldin A  (Figure 6).  All of which have previously been shown to inhibit neurite outgrowth in primary rodent neural cultures.  The authors suggest that this study “supports the hypothesis that measurements of neurite outgrowth are more sensitive than measures of cell health in the context of neurotoxicity screening… and  hN2 cells provide a model in which to investigate the processes of neurite outgrowth in the context of human biology.

Harrill JA, et al. Neurotoxicology. 2010 Jun;31(3):277-90.

hN2™

Back to Top


Transcriptional Profiling

References ArunA Product

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012.

(presentation)

hNP1™

hN2™

Whole genome analysis of human neural stem cells derived from embryonic stem cells and stem and progenitor cells isolated from fetal tissue

Shin S, et al. Stem Cells. 2007 May;25(5):1298-306.

hNP1™

AB2™ ANS™

Stage-specific role for shh in dopaminergic differentiation of human embryonic stem cells induced by stromal cells

Swistowska AM, et al. Stem Cells Dev. 2010 Jan;19(1):71-82.

hNP1™

AB2™ ANS™

Back to Top


Transfection

References ArunA Product

Nonviral Gene Delivery in Neural Progenitors Derived from Human Pluripotent Stem Cells

Dhara, Sujoy K. et. al. Methods in Mol Biology, 2011, Vol. 767, Part 5, 343-354, doi: 10.1007/978-1-61779-201-4_2

hNP1™

AB2™ ANS™

Back to Top

 


 

Review Articles


GPCR

References ArunA Product

Regulation of stem cell pluripotency and differentiation by G protein coupled receptors.

Callihan P, et al. Pharmacol Ther. 2010 Nov 10.  [Epub ahead of print]

 

Back to Top

 

High-Throughput Screening

References ArunA Product

Neural progenitor cells as models for high-throughput screens of developmental neurotoxicity: state of the science.

Breier JM, et al. Neurotoxicol Teratol. 2010 Jan-Feb;32(1):4-15. Epub 2009 Jun 24.

 

Back to Top

 

Neural Differentiation

References ArunA Product

Neural differentiation of human embryonic stem cells.

Dhara SK, Stice SL. J Cell Biochem. 2008 Oct 15;105(3):633-40. Review.

 

Development and differentiation of neural rosettes derived from human embryonic stem cells.

Wilson PG, Stice SS. Stem Cell Rev. 2006;2(1):67-77. Review.

 

Back to Top

 

Synapse Formation

References ArunA Product

Role of astrocytes, soluble factors, cells adhesion molecules and neurotrophins in functional synapse formation: implications for human embryonic stem cell derived neurons.

Dodla MC, et al. Curr Stem Cell Res Ther. 2010 Sep;5(3):251-60.

 

Back to Top

 

 


 

Presentation & Webinars

Society of Toxicology 2012

Advancing the Science of Stem Cells in Toxicology

Stice, Steven L. Society of Toxicology 2012. (presentation)

Back to Top

 


 

Conference Poster Presentations

International Society for Stem Cell Research

2011

Proliferative, Adherent Neural and Mesenchymal Progenitors Derived from Human Induced Pluripotent Stem Cells

Chilton, JM et al. ISSCR 2010

(poster presentation)

2010

 

Generation of Human Induced Pluripotent Stem Cells from Amniotic Epithelial Cells

William L. Blake, Pfizer Corporation, et al ISSCR 2010. (poster presentation)

Back to Top

 

Society of Neuroscience

2011

Development of a novel high throughput screening/high content imaging amenable assay for cell proliferation and migration in human embryonic stem cell derived neural progenitors

Powe, A et al. Society for Neuroscience 2011. (poster presentation)

Back to Top

 

Society of Toxicology

2012

High Content Image Analysis of Neurogenesis Using Human Embryonic Stem Cell Derived Neural Cells

Sirenko, O et al. Society of Toxicology 2012. (poster presentation)

2011

Development of a Novel Assay Platform for Visual Assessment of hESC Derived Neural Progenitor Cell Migration in Response to Neurotoxicants

Powe, A, et al. SOT 2011. (poster presentation)

 

Back to Top